Canada's Next Day DNA Sequencing Service
Flow Genomics is a next-day DNA sequencing provider for services including plasmids, amplicons, End to End amplicons, bacterial genomes, and more. This service is idea for scientists who need their data fast. Flow Genomics web application and submission portal is available at https://app.flowgenomics.com/
Submit your plasmids
Sample requirements
Detailed sample input requirements are available depending the service selected. For plasmids, the minimum submission concentration is 20 ng / µL. We recommend using low-EDTA elution buffer or water when possible.
Recommended DNA quality
- 10 uL of 30 ng/uL in water (by Qubit)
- A260/280 of 1.8 (by Nanodrop)
- A260/230 > 1.8 (by Nanodrop)
Lower quality samples often can be sequenced, but cannot be guaranteed.
Qubit quantification is more accurate than Nanodrop, which tends to over-estimate the concentration resulting in failures. No amplification steps are performed for plasmid sequencing - everything that is in the sample is sequenced. Low-quality samples (low purity, contaminated with genomic DNA or RNA, degraded plasmids) will yield poor data. When samples fail, we attempt re-sequencing once free of charge. We still charge for failed samples since reagents are used on them.
Set up a dropbox
*Learn details and get set up with a drop box at your institution by emailing plasmids@flowgenomics.com!
Sample submission
Please send plasmids in 8-strip tubes when submitting less than 48 samples.
Please courier or bring samples to:
Flow Genomics
Unit 203
1377 The Queensway
Etobicoke, Ontario, M8Z 1T1
(647) 732-9981
Please ship at ambient temperature.
What we send you
We send the following files:
- FASTA of each plasmid assembled
- report that can be opened in browser containing quality information and annotations
Sequencing quality
We sequence plasmids using the Oxford Nanopore Technologies platform. After sequencing to a high read depth, Q50 full plasmid sequences are typically achieved (i.e., 1 error in 10 000). The reminaing errors are typically at homopolymer regions. Here's what Oxford Nanopore says about their quality: https://nanoporetech.com/accuracy
Annotation
Each plasmid is annotated as below (pGem) using pLannotate:
Quality information
Sequencing quality of all reads for each plasmid is plotted for troubleshooting purposes. Ideally, most of the sequencing reads should be full-length plasmids as below (e.g., ~3kb reads is the full pGem plasmid).
Questions? Feedback? Collaborate? Reach out to info@flowgenomics.com.